Research associate, postdoctoral, training opportunity studying the role of sphingolipid metabolism in the pathogenesis of diabetic retinopathy at the Department of Physiology at Michigan State University
We are seeking a research associate, postdoctoral, to investigate the mechanism(s) of diabetes-induced activation of the central enzyme in the sphingolipid pathway, acid sphingomyelinase (ASM) in the retina and retinal endothelial cells.
This NIH-funded position will involve studies of miR-15a as a key regulator of both pro-inflammatory and pro-angiogenic pathways through direct binding and inhibition of ASM, and inhibition of VEGF-A. The position will involve studies to evaluate the regulatory role of miR-15a on (a) ASM activation and inflammatory pathways and (b) VEGF-A production and retinal vascular permeability (i) in vitro using retinal cells associated with diabetic retinopathy (e.g., retinal microvascular endothelial cells, Müller cells and retinal pigment epithelial cells) isolated from the retinas of control and diabetic human donors and treated with miR-15a mimics and inhibitors; and (ii) in vivo using STZ induced diabetes and endothelial cell-specific (Tie-2 promoter-driven) miR-15a overexpressing transgenic mouse (Tie-2 miR-15a TG) models.
In addition, the studies will evaluate the regulatory role of miR-15a on ASM activation, ceramide production and membrane fluidity, and progenitor cell entrapment in the bone marrow using (i) control and diabetic human and rodent CACs treated with miR-15a mimics and inhibitors and (ii) “loss of function” and “gain of function” studies using bone marrow chimeras of miR-15a-/- and Tie-2 miR-15a TG, respectively, made diabetic with STZ. (iii) miR-15a overexpression in human diabetic CACs, CD34+ cells to reduce ASM activity and expression, normalize lipid composition and restore their ability to correct vasodegenerative damage in the retina of diabetic mice.
Candidates should have completed a doctorate level degree and have a strong background in lipid metabolism, and retinal physiology and pathophysiology. The successful candidate should have documented success in research including publications in international journals on relevant topics. Compensation is dependent upon qualifications. The University provides a generous package of fringe benefits.
Doctorate -Lipid metabolism, and retinal physiology
Candidates should have completed a doctorate level degree and have a strong background in lipid metabolism, and retinal physiology and pathophysiology.
The successful candidate should have documented success in research including publications in international journals on relevant topics.
Required Application Materials
Applicants should submit an application package that includes a cover letter, CV, relevant prior publications, code samples, and contact information for three professional references.
Review of applications will begin immediately and continue until the position is filled.
Review of Applications Begins On
Summary of Health Risks
Position may work with animals and human tissue/blood, sharp implements, hazardous chemicals, and biohazard materials.
Michigan State University has been advancing the common good with uncommon will for more than 160 years. One of the top research universities in the world, MSU pushes the boundaries of discovery and forges enduring partnerships to solve the most pressing global challenges while providing life-changing opportunities to a diverse and inclusive academic community through more than 200 programs of study in 17 degree-granting colleges.
Internal Number: 541070
About Michigan State University
Spartans work every day to advance the common good in uncommon ways.Together, we tackle some of the world?s toughest problems to find solutions that make life better?from alternative energy to better food safety to breakthrough medical and environmental applications achieved through rare isotope research.We teach. We explore and we discover. We collaborate and lead. We innovate, inspire, and empower. We achieve our potential and create circumstances that help our students and others achieve theirs.We're good at it, and we've been at it for more than 150 years.The nation?s pioneer land-grant university, MSU began as a bold experiment that democratized higher education and helped bring science and innovation into everyday life. The revolutionary concept became a model for the nation.Today, MSU is one of the top research universities in the world?on one of the biggest, greenest campuses in the nation. Home to nationally ranked and recognized academic, residential college, and service-learning programs, we?re a diverse community of dedicated students and scholars, athletes and artists, scientists and leaders.In ways both practical and profound, we work to create a stronger, more sustainable, and more hopeful future for all.