Research Support - Laboratory/Non-Laboratory, Staff/Administrative
The Research Assistant provides research technical support to the Pharmacology faculty and staff. Incumbent will work with direct supervision on these projects. Will primarily work with mammalian primary cultures and cell lines and will maintain records of completed laboratory work. The Research Assistant will coordinate daily work activities with the Pharmacology Faculty supervisor.
Essential Duties and Responsibilities:
Performs a wide variety of standard research procedures with some supervision.
Graphs and analyzes data with some supervision.
Keeps accurate laboratory notebooks.
Keeps accurate laboratory inventories and orders supplies as needed.
Assists with developing new protocols with supervision.
Assists with literature searches.
Maintains scientific equipment which includes maintaining contact with service representative concerning equipment maintenance and sales representatives concerning ordering equipment and supplies.
Confers with supervisor on a regular basis for assessment of progress and direction of research.
Assists in scheduling of experiments.
Is responsible for all standard maintenance in the lab.
Performs a variety of assays and experimental procedures including but not limited to:
Propagation of mammalian cell lines using sterile/aseptic technique.
Conducts immunological assays including, but not limited to Western blots and ELISA.
Conducts molecular biology assays, such as RT-PCR and real-time PCR.
Prepares media, buffers, immunological, and biological reagents as needed.
Conducts high-throughput pharmacology assays using a Fluorescence Imaging Plate Reader (FLIPR II)
Performs immunostaining, confocal microscopy, and image analysis
Safely handles, records, tracks, and disposes of all hazardous chemicals, and biohazardous materials according to institutional guidelines.
Records and maintains data.
Manages inventory of supplies, and maintains adequate stocks - ordering supplies and equipment in anticipation of need for experiments in the immediate future.
All of above items will require that the Research Assistant will be proficient at many basic laboratory skills to include (but not limited to) sterile technique, calculating molarity, accurate measuring, weighing, accurate pipetting, calculating dilutions and dilution factors, and using a computer for data collection.
Mentor students on their research projects as required.
Other duties as assigned.
This position has no supervisory responsibilities.
To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed below are representative of the knowledge, skill, and/or ability required. Must be able to work in a constant state of alertness and safe manner. Must have regular, predictable, in-person attendance. Reasonable accommodations may be made to enable individuals with disabilities to perform essential functions.
Must have schedule flexibility and be willing to occasionally work late hours or weekends.
Must be willing to work with human cell lines.
Must be willing to work with radioactivity and hazardous chemicals.
Must be willing to work with rodents.
Education and/or Experience:
A Bachelor’s degree in the Biological Sciences or Chemistry and 0-2 years of previous laboratory experience are required. Experience with cell culture and molecular biology techniques is highly desirable.
Computer proficiency in MS Office (Word, Excel, Outlook) required. Experience with Statistics, Graphing and Image software is beneficial.
Intermediate Skills: Ability to read and interpret documents such as safety rules, operating and maintenance instructions and procedural manuals. Ability to communicate effectively in both written and verbal communications with faculty, staff, students, and outside vendors and collaborators.
Intermediate Skills: Ability to apply common sense understanding to carry out instructions furnished in written, oral, or diagram form. Ability to deal with problems involving several concrete variables in standardized situations.
Intermediate Skills: Ability to calculate figures and amounts such as discounts, interests, commissions and proportions, percentages, area, circumference, and volume. Ability to apply concepts of basic algebra and geometry.
The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
Ability to lift 20 lbs.; vision correctable to 20/20 in both eyes; ability to distinguish depth; hearing correctable to normal; ability to tolerate prolonged standing and sitting; use of fingers, hands and arms; unrestricted movement of upper and lower extremities for bending, stretching, reaching, walking and pushing; ability to bend over and touch knees; and ability to tolerate repeated routine tasks.
The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. Individuals will occasionally be exposed to fumes or airborne particles and the risk of radiation. Individuals will be regularly exposed to toxic or caustic chemicals. The noise level in the work environment is usually moderate.
Midwestern University is an Equal Opportunity/Affirmative Action employer that does not discriminate against an employee or applicant based upon race, color, religion, gender, national origin, disability, or veterans status, in accord with 41 C.F.R. 60-1.4(a), 250.5(a), 300.5(a) and 741.5(a). Midwestern University complies with the Smoke-Free Arizona Act (A.R.S. 36-601.01) and the Smoke Free Illinois Act (410 ILCS 82/). Midwestern University complies with the Illinois Equal Pay Act of 2003 and Arizona Equal Pay Acts.
Internal Number: RESEA02178
About Midwestern University
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